| Place | Name | | Affiliation | Time | | | Heat (Pl) |
|---|
| 1 | Candace Hill | 17 | Rockdale County Tc-Ga-Girls | 23.52 | Q | +0.5 | 2 (1) |
| 2 | Diamond Spaulding | 15 | Aquinas Tc-Fl | 23.66 | Q | +0.9 | 1 (1) |
| 3 | Jayla Kirkland | 17 | Una-Al-Birmingha-Hawkins` | 23.72 | Q | +0.2 | 4 (1) |
| 4 | Katlin Sherman | 15 | Mustang Tc-Nc | 23.85 | Q | -0.3 | 5 (1) |
| 5 | Kate Hall | 15 | Una-Me-Casco-Hall | 23.98 | q | +0.9 | 1 (2) |
| 6 | Brenessa Thompson | 15 | Medgar Evers College Prep | 23.99 | q | -0.3 | 5 (2) |
| 7 | Carolyn Brown | 15 | Phoebus Tc-Va | 24.04 | Q | -0.1 | 3 (1) |
| 8 | Brandee' Johnson | 16 | War Tc-Va | 24.05 | q | -0.1 | 3 (2) |
| 9 | Sekayi Bracey | 16 | Kentwood Tc-Mi | 24.10 | | -0.3 | 5 (3) |
| 10 | Serenity Douglas | 16 | Una-Ga-Snellville-Douglas | 24.23 | | +0.2 | 4 (2) |
| 11 | Akua Obeng-Akrofi | 14 | Una-Ga-Lawre-Obeng-Akrofi | 24.24 | | +0.9 | 1 (3) |
| 12 | Janel Pate | 15 | Trainng Ground Tc-Tn | 24.26 | | -0.1 | 3 (3) |
| 13 | Erin Ford | 15 | Fast Lane Tc-Fl | 24.34 | | -0.1 | 3 (4) |
| 14 | Tamara Clark | 17 | High Point Heat-NC | 24.41 | | -0.1 | 3 (5) |
| 15 | Riley Egbula | 15 | Greenvillejets-SC | 24.46 | | -0.1 | 3 (6) |
| 16 | Raven Grant | 15 | Una-Sc-Conway-Thomas | 24.47 | | +0.2 | 4 (3) |
| 17 | Symone Darius | 16 | New Rochelle Tc-Ny | 24.48 | | +0.5 | 2 (2) |
| 18 | Stephanie Ferrante | 14 | Chardon Tc-Oh | 24.57 (24.562) | | -0.1 | 3 (7) |
| 19 | Torie Robinson | 14 | Winslow Elite Tc-Nj | 24.57 (24.568) | | +0.9 | 1 (4) |
| 20 | Cedrina Singletary | 15 | Young Achievers Sc-Fl | 24.63 | | +0.5 | 2 (3) |
| 21 | Caitland Smith | 14 | Una-Al-Birmingham-Adams | 24.65 | | +0.2 | 4 (4) |
| 22 | Kiara Lester | 16 | Deptford Tc-Nj | 24.69 (24.686) | | +0.5 | 2 (4) |
| 23 | Kaysha Love | 16 | Triple Crown Tc-Nv | 24.69 (24.689) | | -0.3 | 5 (4) |
| 24 | Tia Jackson | 15 | JR Tucker Tc-Va | 24.75 (24.744) | | -0.3 | 5 (5) |
| 25 | Bria Saunders | 14 | Una-Nj-Parsippan-Saunders | 24.75 (24.746) | | +0.9 | 1 (5) |
| 26 | Imani Solan | 15 | Suffern Tc-Ny | 24.77 | | +0.5 | 2 (5) |
| 27 | Isis Brooks | 16 | East Forsyth Tc-Nc | 24.81 | | +0.9 | 1 (6) |
| 28 | Morgan Towe | 16 | Kempsville Tc-Va | 24.82 (24.811) | | +0.5 | 2 (6) |
| 29 | Martha Sam | 15 | 5-Star-Blake-Va | 24.82 (24.818) | | +0.2 | 4 (5) |
| 30 | Brittney Thomas | 14 | Trackmyspeed-IL | 25.09 | | +0.5 | 2 (7) |
| 31 | Bryana Robinson | 14 | Una-Ga-Austell-Robinson | 25.11 | | +0.2 | 4 (6) |
| 32 | Jody Knight | 14 | Bentonville Tc-Ar | 25.14 | | -0.3 | 5 (6) |
| 33 | Gabriele Cunningham | 15 | Mallard Creek Tc-Nc | 25.17 | | -0.1 | 3 (8) |
| 34 | Janay Fields | 16 | Track Express Tc-Md | 25.44 | | +0.2 | 4 (7) |
| 35 | Phyllis Gordon | 15 | Neptune Tc-Nj-Girls | 25.76 | | +0.9 | 1 (7) |
| Maiya Dendy | 15 | Lightning Fast Tc-De | DNS | | | 5 |