| Place | Name | | Affiliation | Time | | | Heat (Pl) |
|---|
| 1 | Alexis Duncan | 16 | Running Tigers Tc-Tx | 13.44 | Q | +1.6 | 3 (1) |
| 2 | Sydney McLaughlin | 17 | Union Catholic Tc-Nj | 13.46 | Q | +1.7 | 5 (1) |
| 3 | Ashley Miller | 16 | Una-Co-Greenwood_v-Miller | 13.49 | Q | +2.8 | 4 (1) |
| 4 | Anna Cockrell | 16 | Providence Day School Tc- | 13.53 | Q | +1.6 | 1 (1) |
| 5 | Ebony Williams | 15 | Mustang Tc-Nc | 13.71 | Q | +2.0 | 2 (1) |
| 6 | Jacklyn Howell | 14 | Bulldog Tc-Nc | 13.76 (13.751) | q | +1.6 | 1 (2) |
| 7 | Morgan Knight | 14 | South County Tc-Va | 13.76 (13.758) | q | +2.0 | 2 (2) |
| 8 | Timetria Mitchell | 16 | Northwestern Tc-Fl | 13.88 | q | +1.6 | 3 (2) |
| 9 | Shanel Burr | 14 | The Heat Tc-Ga | 13.90 | | +1.6 | 3 (3) |
| 10 | Brittany Kelly | 14 | Una-Tn-Clarksville-Kelly | 13.99 | | +2.8 | 4 (2) |
| 11 | Natisha Dixon | 14 | Gazelle Tc-Ga | 14.08 | | +1.6 | 1 (3) |
| 12 | Emma Spagnola | 14 | Trackmyspeed Tc-Il | 14.12 | | +2.8 | 4 (3) |
| 13 | Kylie McMillan | 15 | Southern Guilford Tc-Nc | 14.15 (14.143) | | +2.0 | 2 (3) |
| 13 | Tonea Marshall | 16 | Running Tigers Tc-Tx | 14.15 (14.143) | | +1.7 | 5 (2) |
| 15 | Breanne Bygrave | 16 | Jr Striders T+F Club-NC | 14.20 | | +1.6 | 1 (4) |
| 16 | Ashley Wiggins | 14 | New Rochelle Tc-Ny | 14.23 (14.226) | | +1.7 | 5 (3) |
| 17 | Kelsey Heyward | 16 | Greenvillejets-SC | 14.23 (14.228) | | +2.8 | 4 (4) |
| 18 | Aspen Gaita | 15 | Una-Pa-East_strouds-Gaita | 14.42 | | +1.6 | 3 (4) |
| 19 | Sara Gardner | 14 | Delaware Valley Tc-Nj | 14.43 (14.422) | | +1.6 | 1 (5) |
| 20 | Ashley Edwards | 15 | Hounds Tc-Nj | 14.43 (14.428) | | +1.6 | 1 (6) |
| 21 | leann parke | 14 | Harry S. Truman Tc-Ny | 14.43 (14.429) | | +1.7 | 5 (4) |
| 22 | Jessica Lee | 14 | Una-Nj-Skillman-Lee | 14.44 | | +1.6 | 3 (5) |
| 23 | Kyra Atkins | 15 | Greenvillejets-SC | 14.48 | | +1.7 | 5 (5) |
| 24 | McKenna Mattson | 14 | Jenison Tc-Ga | 14.49 (14.485) | | +2.8 | 4 (5) |
| 25 | Breann Burns | 14 | Pulaski Academy Tc-Ny | 14.49 (14.488) | | +2.0 | 2 (4) |
| 26 | Alexus Pyles | 16 | Una-Md-Clarksburg-Pyles | 14.50 | | +1.6 | 3 (6) |
| 27 | Nyah Hines | 15 | Garner Tc-Nc | 14.56 | | +2.8 | 4 (6) |
| 28 | Anna Dean | 15 | Hillsboro Tc-Or | 14.69 | | +2.8 | 4 (7) |
| 29 | Keri Ertel | 15 | Bloomington North Tc-In | 14.73 | | +1.6 | 1 (7) |
| 30 | Morgan Robertson | 14 | Run Northville Tc-Mi | 15.14 | | +1.7 | 5 (6) |
| 31 | Dominique Sullivan | 14 | Decatur Tc-Ga | 15.20 | | +1.6 | 3 (7) |
| 32 | Chantel Ray | 14 | Hampton Tc-Va | 16.83 | | +2.0 | 2 (5) |
| Athena Carpenter | 14 | Titans Tc-Ga | DNS | | | 1 |
| Marinice Bauman | 14 | Una-Oh-Galena-Bauman | DNS | | | 2 |
| Jordan Fields | 16 | St. Johns Striders-FL-Girls | DNS | | | 2 |
| Gabriele Cunningham | 15 | Mallard Creek Tc-Nc | DNF | | | 5 |
| Shayla Broughton | 16 | Medgar Evers College Prep | DNS | | | 5 |