| Place | Name | | Affiliation | Time | | | Heat (Pl) |
|---|
| 1 | Hunter Koike | 14 | St. Johns Striders-FL-Boys | 50.65 | | | 3 (1) |
| 2 | Benjamin Stewart | 14 | North Kingstown Tc-Ri | 50.80 | | | 1 (1) |
| 3 | Matt Ludwig | 15 | Una-Oh-Chardon-Ludwig | 51.06 | | | 3 (2) |
| 4 | Andris Sturans | 14 | Chantilly Tc-Va | 51.16 (51.152) | | | 2 (1) |
| 5 | Matt Fischetti | 14 | Phillips Academy Tc-Ma | 51.16 (51.156) | | | 3 (3) |
| 6 | Isaac Agyemang | 15 | Woodbridge Tc-Va | 52.25 | | | 1 (2) |
| 7 | Christian Friis | 16 | Una-Fl-Santa_rosa_b-Friis | 52.47 | | | 2 (2) |
| 8 | Tim Schanstra | 14 | Pope John Xxiii Tc-Nj | 53.25 | | | 1 (3) |
| 9 | Nick Fofana | 14 | Nitehawks New England Ath | 54.09 | | | 1 (4) |
| 10 | Benjamin Brunson | 15 | Middle Tn Elite Tc-Tn | 54.63 | | | 1 (5) |
| 11 | Rodney Ripley | 14 | Una-Tx-Spring-Ripley | 54.86 | | | 2 (3) |
| 12 | Hayden Ashley | 16 | Personal Best Athletics-O | 55.62 | | | 1 (6) |
| 13 | Dylan Altland | 14 | Una-Nj-Swedesboro-Altland | 55.78 | | | 3 (4) |
| 14 | Ben Hester | 14 | Kentwood Tc-Mi | 55.82 | | | 2 (4) |
| 15 | William Ackerman | 14 | Arlington Tc-Ny | 55.98 | | | 2 (5) |
| 16 | Matthew Hollis | 14 | Stony Brook Tc-Ny | 56.09 | | | 3 (5) |
| 17 | Dante Newberg | 15 | Una-Fl-Valrico-Newberg | 56.32 | | | 2 (6) |
| 18 | Dylan Smith | 15 | Slo Town Tc-Ca | 59.92 | | | 3 (6) |
| Jacob McKinnon | 14 | Patriot Pole Vault Club-M | DNS | | | 2 |
| Ryan Lipe | 14 | Middle Tn Elite Tc-Tn | DNF | | | 3 |