| Place | Name | | Affiliation | Time | | | Heat (Pl) |
|---|
| 1 | Karimah Davis | 17 | Fast Lane Tc-Fl | 24.26 | Q | +1.9 | 2 (1) |
| 2 | Taylor DeLoach | 16 | St Vincent Academy Tc-Ga | 24.48 | Q | +1.9 | 4 (1) |
| 3 | Isabella DiMare | 15 | Notre Dame Academy Tc-Ma | 24.55 | q | +1.9 | 2 (2) |
| 4 | Atlanta Woodall | 15 | Riverside Tc-Nc | 24.70 | Q | +1.2 | 1 (1) |
| 5 | Tamani Wilson | 16 | Una-Fl-Tallahassee-Wilson | 24.72 | Q | +2.2 | 3 (1) |
| 6 | Lauren Brzozowski | 15 | Ramapo Tc-Nj-Girls | 24.73 | q | +1.9 | 4 (2) |
| 7 | Lauren White | 16 | Una-Nc-Raleigh-White | 24.74 | q | +1.9 | 2 (3) |
| 8 | Jamie Stacey | 15 | Track Eastern Carolina-NC | 24.76 | q | +2.2 | 3 (2) |
| 9 | Alexis Harrell | 15 | Northwestern Tc-Fl | 24.87 | | +1.9 | 4 (3) |
| 10 | Delaney Wickman | 15 | South Lakes Tc-Va | 24.92 | | +1.2 | 1 (2) |
| 11 | Courtney Blanden | 14 | Charlotte Flights Tc-Nc | 24.98 (24.971) | | +2.2 | 3 (3) |
| 12 | Kiana Robinson | | Velocity Track Academy-GA | 24.98 (24.975) | | +1.2 | 1 (3) |
| 13 | Amanda Crawford | 16 | Paul Robeson Tc-Ny | 25.03 | | +1.9 | 4 (4) |
| 14 | Deborah Olawuni | 14 | Una-Md-Takoma_par-Lockard | 25.16 | | +1.2 | 1 (4) |
| 15 | Margaret Sullivan | 14 | Una-Md-Hagerstow-Sullivan | 25.27 | | +2.2 | 3 (4) |
| 16 | Tori Ray | 15 | Hampton Tc-Va | 25.33 | | +1.2 | 1 (5) |
| 17 | Lakin Hatcher | 15 | McDonogh School Tc-Md | 25.40 | | +2.2 | 3 (5) |
| 18 | Courtney Warner | 16 | Track Eastern Carolina-NC | 25.45 | | +1.9 | 4 (5) |
| 19 | Ashlan Rosier | | Velocity Track Academy-GA | 25.73 | | +1.9 | 2 (4) |
| 20 | Cameren Pryor | 15 | Millbrook Tc-Nc | 25.74 | | +2.2 | 3 (6) |
| 21 | Alexis Pierre-Antoine | 16 | Illinois Elite Youth Tc-I | 25.93 | | +1.9 | 4 (6) |
| Niata Alexander | 14 | Titans Tc-Ga | DNS | | | 1 |
| Janee Nicholas | 16 | Track Phi Track Elite-GA | DNS | | | 1 |
| Payton Ashe | 14 | Una-Fl-Gainesville-Ashe | DNS | | | 2 |
| Miah Webb | 15 | Oaks Christian Tc-Ca | DNS | | | 2 |
| Alex Council | 15 | Bull City Express-NC | DNS | | | 2 |
| Shalon Conley | 14 | Pickerington Tc-Oh | DNS | | | 3 |
| Sophia Falco | 17 | Lakewood Ranch Tc-Fl | DNS | | | 4 |