| Place | Name | | Affiliation | Time | | | Heat (Pl) |
|---|
| 1 | Emma Gallagher | 15 | Garden City Tc-Ny | 55.40 | | | 4 (1) |
| 2 | Eizabeth Adesanya | 14 | 5-Star-Blake-Va | 56.08 | | | 3 (1) |
| 3 | Chelsea Zoller | 16 | Gazelle Tc-Ga | 56.16 | | | 3 (2) |
| 4 | Bryana Robinson | 14 | Una-Ga-Austell-Robinson | 56.26 | | | 5 (1) |
| 5 | Johnyce Powell | 15 | Motor City Tc-Mi | 56.28 | | | 3 (3) |
| 6 | Mikaila Amerantes | 15 | Mansfield Tc-Ma | 56.42 (56.415) | | | 5 (2) |
| 7 | Dnia Freeman | 16 | Riverdale Baptist Tc-Md | 56.42 (56.420) | | | 4 (2) |
| 8 | Delaney Wickman | 15 | South Lakes Tc-Va | 56.57 | | | 5 (3) |
| 9 | McKinley McNeill | 16 | Mustang Tc-Nc | 56.62 | | | 5 (4) |
| 10 | Ersula Farrow | 15 | Una-Mi-Grosse_poin-Farrow | 56.79 | | | 3 (4) |
| 11 | Tia Jackson | 15 | JR Tucker Tc-Va | 56.99 | | | 3 (5) |
| 12 | Olivia Jamison | 15 | Pennsauken Tc-Nj | 57.01 | | | 4 (3) |
| 13 | Maddy Berkson | 14 | Classical Tc-Ri | 57.09 | | | 1 (1) |
| 14 | Dominique Watkins | 14 | Abraham Clark Tc-Nj | 57.23 | | | 3 (6) |
| 15 | Tia Robinson | 15 | Jr Striders T+F Club-NC | 57.32 | | | 4 (4) |
| 16 | Aubree Collins | 14 | Quicksilver Tc-Ga | 57.38 | | | 4 (5) |
| 17 | Jasmin Allen | 14 | Bulldog Tc-Nc | 57.40 | | | 4 (6) |
| 18 | Kiana Lee | 15 | Irvington Tc-Ny | 57.41 | | | 5 (5) |
| 19 | Zuri Davis | 14 | War Eagles Tc-Ga | 57.44 | | | 1 (2) |
| 20 | Nichelle Scott | 14 | LC Bird Hs-Va | 57.49 | | | 3 (7) |
| 21 | Anna Curlin | 14 | Western Hills Tc-Ky | 57.77 (57.761) | | | 4 (7) |
| 22 | Torisha LaForce | 15 | Una-Ny-Queens-Laforce | 57.77 (57.764) | | | 1 (3) |
| 23 | Brooke Stith | 17 | Brunswick Tc-Va | 58.03 | | | 1 (4) |
| 24 | Kristoni Barnes | 16 | Carolina Elite T+F-Nc | 58.07 | | | 2 (1) |
| 25 | Brianna Belo | 14 | The Episcopal Academy Tc- | 58.13 | | | 4 (8) |
| 26 | Jocelyn Sharpe | 15 | The Flying Js-OH | 58.37 | | | 2 (2) |
| 27 | JeMay Ward | 15 | Jr Striders T+F Club-NC | 58.94 | | | 2 (3) |
| 28 | Sydney Davis | 15 | Grayson Tc-Ga | 59.32 | | | 2 (4) |
| 29 | Shakai Ward | 15 | Una-Nc-Roaring_river-Ward | 59.37 | | | 2 (5) |
| Jinjer Pearce | 16 | Rye Country Day School TC | DNS | | | 1 |
| Deja Parrish | 14 | Una-Fl-Fort_laude-Parrish | DNS | | | 1 |
| Christina Dixon | 14 | South Central Tc-Nc | DNS | | | 1 |
| Jasmine Hill | 14 | Mount Vernon Tc-Va | DNS | | | 2 |
| Payton Ashe | 14 | Una-Fl-Gainesville-Ashe | DNS | | | 2 |
| Shannon Quinn | 15 | Una-Pa-Carlisle-Quinn | DNS | | | 3 |
| Karimah Davis | 17 | Fast Lane Tc-Fl | DNS | | | 5 |
| Lauren Jackson | 16 | Sprint Athletics Tc-Ga | DNS | | | 5 |
| Janee Nicholas | 16 | Track Phi Track Elite-GA | DNS | | | 5 |