| Place | Name | | Affiliation | Time | | | Heat (Pl) |
|---|
| 1 | Margaret Sullivan | 14 | Una-Md-Hagerstow-Sullivan | 14.29 | Q | +1.9 | 2 (1) |
| 2 | Courtney Nelson | 14 | Personal Best Athletics-O | 14.34 | Q | +1.6 | 1 (1) |
| 3 | Ashley Williams | 14 | Easton Area Tc-Pa | 14.53 | Q | +0.9 | 3 (1) |
| 4 | Arbria Williams | 14 | Withrow Tc-Oh | 14.55 | Q | -1.2 | 4 (1) |
| 5 | Brianna Holloway | 16 | Motor City Tc-Mi | 14.57 | q | +1.6 | 1 (2) |
| 6 | Kayla Moore | 17 | Durham Striders TC | 14.68 | q | +1.9 | 2 (2) |
| 7 | Alliyah Stevens | 15 | Mount Vernon Tc-Va | 14.79 | q | +0.9 | 3 (2) |
| 8 | Ariel Davis | 15 | Platinum Sports Academy-N | 14.91 | q | -1.2 | 4 (2) |
| 9 | Taylor Murphy | 15 | Bernards Tc-Nj-Girls | 14.97 | | +1.9 | 2 (3) |
| 10 | Aviana Goode | 16 | Bay Shore Tc-Ny | 14.98 (14.971) | | +1.6 | 1 (3) |
| 11 | Amanda Moroney | 14 | Pearl River Tc-Ny-Girls | 14.98 (14.979) | | -1.2 | 4 (3) |
| 12 | Helnsarah Penda | 16 | Young Gunz-MD | 15.02 | | +1.6 | 1 (4) |
| 13 | Mackenzie Aydelette | 15 | St. Johns Striders-FL-Girls | 15.14 | | +1.9 | 2 (4) |
| 14 | Trinity Clements | 15 | Bloomington North Tc-In | 15.16 | | -1.2 | 4 (4) |
| 15 | Nikari Carota | 14 | Adirondack Runners-NY | 15.20 | | -1.2 | 4 (5) |
| 16 | Alexis Pierre-Antoine | 16 | Illinois Elite Youth Tc-I | 15.28 | | +1.6 | 1 (5) |
| 17 | Ashley Pryce | 14 | Stow-Munroe Falls Tc-Oh-Girls | 15.30 | | +0.9 | 3 (3) |
| 18 | Kailsi Latta-Thompson | 15 | Motor City Tc-Mi | 15.38 | | +1.9 | 2 (5) |
| 19 | Makaila Roberts | 15 | Ronald Reagan Tc-Nc | 15.40 | | +0.9 | 3 (4) |
| 20 | Ijaz McCallum | 15 | US Elite Intl Jrs Tc-Md | 15.41 | | +1.6 | 1 (6) |
| 21 | Onyx Simpson | 15 | On Your Mark Sport-GA | 15.56 | | +0.9 | 3 (5) |
| 22 | Ellie Randolph | 15 | Norfolk Academy Tc-Va | 15.57 | | +0.9 | 3 (6) |
| 23 | Ellie Wiedeman | 14 | Springboro Tc-Oh | 15.81 | | +1.9 | 2 (6) |
| 24 | Kristen Laidlaw | 17 | Aquinas Tc-Fl | 15.97 | | +1.9 | 2 (7) |
| Bria Stith | 16 | Brunswick Tc-Va | DNF | | | 2 |
| Jessica Watson | 15 | Ronald Reagan Tc-Nc | DNS | | | 4 |