| Place | Name | | Affiliation | Time | | | Heat (Pl) |
|---|
| 1 | Kentwood Tc-Mi | | 811 A | 48.29 | Q | +1.4 | 1 (1) |
| 2 | Notre Dame Academy Tc-Ma | | 1229 A | 48.67 | Q | +2.7 | 4 (1) |
| 3 | Stone Bridge Tc-Va | | 1090 A | 49.01 | q | +1.4 | 1 (2) |
| 4 | Cosby Tc-Va | | 1671 A | 49.17 | Q | +1.2 | 3 (1) |
| 5 | Andover Warrior Tc-Ma | | 1568 A | 49.22 | Q | +0.2 | 2 (1) |
| 6 | Liberty Tc-Md | | 564 A | 49.30 | q | +0.2 | 2 (2) |
| 7 | Sweet Home Tc-Ny | | 1618 A | 49.31 | Q | +1.6 | 5 (1) |
| 8 | Arundel Tc-Md | | 617 A | 49.33 (49.322) | | +0.2 | 2 (3) |
| 9 | Spirit of Pre Tc-Mi-Girls | | 533 A | 49.33 (49.329) | | +2.7 | 4 (2) |
| 10 | Kinetic Rc-Ny | | 1184 A | 49.41 | | +1.6 | 5 (2) |
| 11 | Dunbar Tc-Dc | | 708 A | 49.44 | | +1.4 | 1 (3) |
| 12 | Jr Striders T+F Club-NC | | 1639 A | 49.48 | | +1.6 | 5 (3) |
| 13 | Nova Tc-In | | 473 A | 49.50 | | +2.7 | 4 (3) |
| 14 | Oakton Tc-Va | | 612 A | 49.53 | | +1.6 | 5 (4) |
| 15 | Top of the Hill Tc-Nj | | 1131 A | 49.57 | | +1.2 | 3 (2) |
| 16 | Daniel Hand Tc-Ct-Girls | | 1174 A | 49.68 | Q | +0.9 | 6 (1) |
| 17 | Harry S. Truman Tc-Ny | | 970 A | 49.73 | | +2.7 | 4 (4) |
| 18 | Damascus Tc-Md | | 1387 A | 49.79 (49.782) | | +1.4 | 1 (4) |
| 19 | Maryland Jaguars-MD | | 571 A | 49.79 (49.783) | | +0.2 | 2 (4) |
| 20 | Hardin Valley Academy Tc- | | 1227 A | 49.79 (49.786) | | +1.4 | 1 (5) |
| 21 | Paul Robeson Tc-Ny | | 777 A | 49.82 | | +0.9 | 6 (2) |
| 22 | The Episcopal Academy Tc- | | 1320 A | 49.85 | | +1.2 | 3 (3) |
| 23 | Wild Kitten Ac-Ma | | 1504 A | 49.87 | | +1.2 | 3 (4) |
| 24 | Bloomfield Tc-Ct-Girls | | 280 A | 49.92 | | +1.2 | 3 (5) |
| 25 | War Tc-Va | | 299 A | 50.06 | | +0.9 | 6 (3) |
| 26 | Reservoir Tc-Md | | 524 A | 50.08 | | +1.2 | 3 (6) |
| 27 | Scarsdale Tc-Ny-Girls | | 1370 A | 50.20 | | +0.9 | 6 (4) |
| 28 | School of the Arts Tc-Ny- | | 1668 A | 50.27 | | +1.6 | 5 (5) |
| 29 | Titans Tc-Ma | | 1155 A | 50.28 | | +2.7 | 4 (5) |
| 30 | Lakewood Ranch Tc-Fl | | 262 A | 50.32 | | +0.2 | 2 (5) |
| 31 | Granby Tc-Va | | 1454 A | 50.35 | | +2.7 | 4 (6) |
| 32 | Bishop McGann Mercy Tc-Ny | | 108 A | 50.37 | | +1.2 | 3 (7) |
| 33 | JR Tucker Tc-Va | | 655 A | 50.52 | | +2.7 | 4 (7) |
| 34 | Bernards Tc-Nj-Girls | | 414 A | 50.53 | | +0.9 | 6 (5) |
| 35 | East Brunswick Tc-Nj-Girl | | 1034 A | 50.64 | | +1.4 | 1 (6) |
| 36 | East Haven Tc-Ct | | 346 A | 50.93 | | +1.4 | 1 (7) |
| 37 | Manchester Tc-Va | | 322 A | 51.13 | | +0.9 | 6 (6) |
| 38 | Red Bank Regional Tc-Nj | | 1543 A | 51.20 | | +1.4 | 1 (8) |
| 39 | The Potomac School Tc-Va | | 1132 A | 51.43 | | +1.6 | 5 (6) |
| 40 | Maryvale Prep Tc-Md | | 995 A | 52.02 | | +0.2 | 2 (6) |
| 41 | McKinley Tech Tc-Dc | | 1161 A | 57.29 | | +1.6 | 5 (7) |
| 42 | Millbrook Tc-Nc | | 1358 A | 57.53 | | +1.6 | 5 (8) |
| Penfield Tc-Ny | | 522 A | DNS | | | 2 |
| River Dell Tc-Nj | | 767 A | DQ | | | 6 |